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Infection and Immunity, February 2003, p. 1016-1019, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.1016-1019.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Influence of Extracellular Bactericidal Agents on Bacteria within Macrophages

Terri S. Hamrick,{dagger}* Adam H. Diaz, Edward A. Havell, John R. Horton, and Paul E. Orndorff

Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606

Received 17 June 2002/ Returned for modification 16 October 2002/ Accepted 14 November 2002

We employed gentamicin-sensitive and -resistant derivatives of Escherichia coli in a macrophage phagocytosis assay that compared {lambda} bacteriophage and gentamicin as extracellular bactericidal agents. Colony counts and direct microscopic examination of phagocytized E. coli supported the conclusion that gentamicin entered macrophages, even at low concentrations, and contributed to their bactericidal activity. Also, two E. coli strains differing in the ability to express the adhesin of type 1 pili (FimH) were distinguishably different in intracellular survival when {lambda} was used as the extracellular killing agent but were indistinguishable when gentamicin was employed.

* Corresponding author. Mailing address: College of Veterinary Medicine, 4700 Hillsborough St., Raleigh, NC 27606. Phone: (919) 513-6207. Fax: (919) 513-6455. E-mail: paul_orndorff{at}ncsu.edu.

Editor: B. B. Finlay

{dagger} Present address: Department of Pharmaceutical Sciences, Campbell University School of Pharmacy, Buies Creek, NC 27506.

Infection and Immunity, February 2003, p. 1016-1019, Vol. 71, No. 2
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.2.1016-1019.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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