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Infection and Immunity, May 2001, p. 2872-2877, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2872-2877.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Staphylococcus aureus Fibronectin Binding Proteins Are Essential for Internalization by Osteoblasts but Do Not Account for Differences in Intracellular Levels of Bacteria

Saddif Ahmed,1 Sajeda Meghji,1 Rachel J. Williams,1 Brian Henderson,1 Jeremy H. Brock,2 and Sean P. Nair1,*

Cellular Microbiology Research Group, Eastman Dental Institute, University College London,1 and Department of Immunology, Western Infirmary, University of Glasgow, Glasgow,2 United Kingdom

Received 27 November 2000/Returned for modification 3 January 2001/Accepted 6 February 2001

Staphylococcus aureus is a major pathogen of bone that has been shown to be internalized by osteoblasts via a receptor-mediated pathway. Here we report that there are strain-dependent differences in the uptake of S. aureus by osteoblasts. An S. aureus septic arthritis isolate, LS-1, was internalized some 10-fold more than the laboratory strain 8325-4. Disruption of the genes for the fibronectin binding proteins in these two strains of S. aureus blocked their ability to be internalized by osteoblasts, thereby demonstrating the essentiality of these genes in this process. However, there were no differences in the capacity of these two strains to bind to fibronectin or osteoblasts. Analysis of the kinetics of internalization of the two strains by osteoblasts revealed that strain 8325-4 was internalized only over a short period of time (2 h) and to low numbers, while LS-1 was taken up by osteoblasts in large numbers for over 3 h. These differences in the kinetics of uptake explain the fact that the two strains of S. aureus are internalized by osteoblasts to different extents and suggest that in addition to the fibronectin binding proteins there are other, as yet undetermined virulence factors that play a role in the internalization process.

* Corresponding author. Mailing address: Cellular Microbiology Research Group, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. Phone: 44 (0)20 7915 1118. Fax: 44 (0)20 7915 1127. E-mail: snair{at}eastman.ucl.ac.uk.

Infection and Immunity, May 2001, p. 2872-2877, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2872-2877.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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