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Infect Immun, July 1998, p. 3179-3182, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Immunization with Pneumocystis carinii gpA Is Immunogenic but Not Protective in a Mouse Model of P. carinii Pneumonia

Francis Gigliotti,1,* James A. Wiley,2 and Allen G. Harmsen2

University of Rochester School of Medicine, Rochester, New York 14642,1 and The Trudeau Institute, Saranac Lake, New York 129832

Received 9 February 1998/Returned for modification 14 March 1998/Accepted 8 April 1998

Immunization with whole Pneumocystis carinii has been shown to protect mice from the development of P. carinii pneumonia (PCP) when they are subsequently immunosuppressed and challenged with viable organisms. To determine whether these results could be duplicated by using a subunit vaccine, we examined the immunogenicity and efficacy of an immunization strategy based on P. carinii gpA. This antigen was chosen for study because passive immunoprophylaxis, based on gpA, has been shown to be partially protective in various animal models of infection. Immunization with gpA produced an anti-gpA specific antibody response comparable to that resulting from immunization with whole organisms. However, in contrast to immunization with whole P. carinii, which was protective, immunization with gpA did not protect T-cell-depleted mice from the development of PCP. These studies suggest that other antigens in addition to gpA need to be evaluated for their role in protective immunity against P. carinii.

* Corresponding author. Mailing address: University of Rochester School of Medicine, 601 Elmwood Ave., Box 690, Rochester, NY 14642. Phone: (716) 275-5944. Fax: (716) 273-1104. E-mail: fgig{at}uhura.cc.rochester.edu.

Infect Immun, July 1998, p. 3179-3182, Vol. 66, No. 7
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wells, J., Haidaris, C. G., Wright, T. W., Gigliotti, F. (2006). Active Immunization against Pneumocystis carinii with a Recombinant P. carinii Antigen. Infect. Immun. 74: 2446-2448 [Abstract] [Full Text]  
  • Wells, J., Gigliotti, F., Simpson-Haidaris, P. J., Haidaris, C. G. (2004). Epitope Mapping of a Protective Monoclonal Antibody against Pneumocystis carinii with Shared Reactivity to Streptococcus pneumoniae Surface Antigen PspA. Infect. Immun. 72: 1548-1556 [Abstract] [Full Text]  
  • Gigliotti, F., Harmsen, A. G., Wright, T. W. (2003). Characterization of Transmission of Pneumocystis carinii f. sp. muris through Immunocompetent BALB/c Mice. Infect. Immun. 71: 3852-3856 [Abstract] [Full Text]  
  • Stringer, J. R., Keely, S. P. (2001). Genetics of Surface Antigen Expression in Pneumocystis carinii. Infect. Immun. 69: 627-639 [Abstract] [Full Text]  
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