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Infection and Immunity, November 2009, p. 4940-4946, Vol. 77, No. 11
0019-9567/09/$08.00+0     doi:10.1128/IAI.01420-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Borrelia burgdorferi BmpA Is a Laminin-Binding Protein{triangledown}

Ashutosh Verma,{dagger} Catherine A. Brissette,{dagger} Amy Bowman, and Brian Stevenson*

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky 40536

Received 19 November 2008/ Returned for modification 2 January 2009/ Accepted 16 August 2009

The Borrelia burgdorferi BmpA outer surface protein plays a significant role in mammalian infection by the Lyme disease spirochete and is an important antigen for the serodiagnosis of human infection. B. burgdorferi adheres to host extracellular matrix components, including laminin. The results of our studies indicate that BmpA and its three paralogous proteins, BmpB, BmpC, and BmpD, all bind to mammalian laminin. BmpA did not bind mammalian type I or type IV collagens or fibronectin. BmpA-directed antibodies significantly inhibited the adherence of live B. burgdorferi to laminin. The laminin-binding domain of BmpA was mapped to the carboxy-terminal 80 amino acids. Solubilized collagen inhibited BmpA-laminin binding, suggesting interactions through the collagen-binding domains of laminin. These results, together with previous data, indicate that BmpA and its paralogs are targets for the development of preventative and curative therapies for Lyme disease.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, MS 421 Chandler Medical Center, Lexington, KY 40536-0298. Phone: (859) 257-9358. Fax: (859) 257-8994. E-mail: brian.stevenson{at}uky.edu

{triangledown} Published ahead of print on 24 August 2009.

Editor: J. B. Bliska

{dagger} A. Verma and C. A. Brissette contributed equally to this work.

Infection and Immunity, November 2009, p. 4940-4946, Vol. 77, No. 11
0019-9567/09/$08.00+0     doi:10.1128/IAI.01420-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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